BACTERIAS DEGRADADORAS DE HIDROCARBUROS AISLADAS EN SUELOS CONTAMINADOS DE PETROLEO Aislar cepas bacterianas y. Aislamiento de bacterias potencialmente degradadoras de petróleo en hábitats de ecosistemas costeros en la Bahía de Cartagena, Colombia. Download Citation on ResearchGate | SELECCIÓN DE BACTERIAS CON CAPACIDAD DEGRADADORA DE HIDROCARBUROS Estudio y selección de bacterias aerobias degradadoras de hidrocarburos del petróleo aisladas de costas.

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In general, biodegradation is expected to be more extensive in aliphatic hydrocarbons, which are far more amenable to this process than their aromatic counterparts [35, 47, 48]. Bacterias marinas con capacidad antimicrobiana aisladas de moluscos bivalvos en cultivos. Received in October, Lastly, the genera Kurthia and Azotobacter were represented each by a single bacteriaw. Bacterial metabolism is readjusted as culture ages and less complex substrates saturated linear alkanes and low molecular weight aromatic compounds baxterias exhausted, degraddadoras towards the synthesis of enzyme complexes geared towards the degradation of more complex molecules.

Pure cultures of these strains were further used in crude oil biodegradability assays. Microbial Transformation and degradation of toxic organic chemicals. They arrived to the laboratory for further processing within the first 24 h after their collection. Hydrocarbons are lipophilic compounds that inhibit growth when present at high concentrations [45], inducing petrolwo bacterial stress response and a series of changes at the membrane, enzyme and protein levels [44, 46].

In addition, other authors have shown that the biotransformation of asphaltenes and resins leads to the accumulation of simpler saturated and aromatic derivates, increasing the concentration of these fractions [53, 54].

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The concentration of associated hydroxyl groups OH -cm -1 increased for all treatments, as did that of carboxylic groups cm The cultures were examined every 24 h, selecting all strains exhibiting vigorous growth within a seven day period for further study. A microbiological survey of the western Cuban continental shelf found this species in both northern and southern locations [27].


Online J Biol Sci.

Taxonomy of aerobic marine eubacteria. Monocyclic and polycyclic aromatic hydrocarbon fractions were analyzed by FTIR spectroscopy, as described in Materials and Methods. Figure 3 shows the chromatographic profile of the saturated hydrocarbon fraction, in a signal intensity pico-amperes versus time chart for strains F9S, F10S1 and F1FLC. Biodegradability of Venezuela crude oils. When comparing the degradation of asphaltenes with that of saturated and aromatic hydrocarbons regarding the time point at which they first become detectable as well as their kinetics, it is useful to take into account the environmental characteristics of the habitat from which the microorganisms under examination have been isolated; that is, their environmental adaptations.

Biodegradation of Polycyclic Aromatic Hydrocarbons. Regardless, Joseph et al. Environmental biotechnology techniques have been readily used to remediate ecological disasters caused by large oil spills, such as those caused by the collision of tanker Exxon Valdez with Bligh Reef at Prince William Sound, spilling some 50 tons of crude oil in March [6], and the sinking of Prestigewhich spilled 63 tons of crude and ended up affecting 1 km of French and Spanish coastlines in November [7].

Asphaltenes were precipitated with n-pentane. Depth-resolved quantification of anaerobic toluene degraders and aquifer microbial community patterns in distinct redox zones of a tar oil contaminant plume.

Comparative physiology of phenanthrene degradation by two dissimilar pseudomonads isolated from a creosote-contaminated soil.

Bioremediation of petroleum-and creosote-contaminated soils: Strain isolation The primary selection process employed petorleo different discrimination protocols, denominated here protocol A modified from [8] and B modified from [9]. These authors attributed such a phenomenon to adaptations of these microorganisms to the chronic contamination of their original habitats. The increased levels of phenols and phenoxides may be directly related to the accumulation of end compounds produced by the degradation of resins and asphaltenes.

Strain F10S1 degraded Molecular analysis of surfactant-driven microbial population shifts in hydrocarbon-contaminated soil. Effects of heavy fuel oil on the bacterial community structure of a pristine microbial mat. The cultures were discarded after 15 days. Most research on bioremediation technology has focused therefore on aerobic heterotrophic ddegradadoras, due not only to the taxonomic diversity of hydrocarbon-degrading representatives from this group, but to their ability to use xenobiotic compounds as carbon source in pure cultures [3].



Enrichment, isolation, and phylogenetic identification of polycyclic aromatic hydrocarbon-degrading bacteria from Elizabeth River sediments. Appl Environ Microbiol ;63 9: Measures taken after the Exxon Valdez spill included the addition of nutrients in the form of fertilizers Inipol EAP 22 and Customblerwhich increased the rate of oil bacteriad by three-fold [6]. Two of the isolated strains belonged to the Pseudomonas genus.

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Effects of rhamnolipid biosurfactants on removal of phenanthrene from soil. Trials of bioremediation on a beach affected by the heavy oil spill of the Prestige. Las potencialidades biodegradadoras de estos microorganismos en la limpieza de costas marinas han generado nuevos estudios. Biodegradation of crude oil contaminating marine shorelines and freshwater wetlands.

Molecular characterization of a toluene-degrading methanogenic petrileo. Several authors have pointed out that short chain aliphatic hydrocarbons usually volatilize during the first hours after petrileo spill. Anaerobic microorganisms, however, are less versatile regarding their growth substrate and often display increased sensitivity toward heavy metals, hence playing a smaller role in biodegradation [2]. The Utilization of Certain Hydrocarbons by Microorganisms.

Some authors place some species of this genus into Alteromonas instead [17]. F9S exhibited the best results regarding the degradation of asphaltenes. Accepted for publication in January, Spill Sci technol Bull.